In 2015 the original diet was formulated, and efficiency was measured in a monthly weigh-in of Angelica. As she began to gain weight, the diet was edited to be better equipped for her. After the year, the core diet was finalized as Angelica reached and maintained a healthy weight.
In 2017 I wanted to test my diet further now that I had data supporting the increase in growth rate to ensure I was not causing underlying health conditions, specifically diabetes. I once again contacted the Pennsylvania Society of Biomedical Research and received another grant for more female albino rats. This three-month-long trial involved eight rats split into four groups similar to the first rodent trials. This trial recorded both weight gain at ten points throughout the trial and blood glucose concentrations at three points. Blood glucose concentrations were recorded at the beginning, end, and midpoint (before experimental group diet transition) to see if diets cause hyper or hypoglycemia. After the project, there was not enough data to draw any clear conclusions on the effects of the diet on blood glucose levels, but the data did point to my diet allowing for better maintenance of a healthy blood glucose concentration compared to the store-bought diet.
After the extensive research conducted on rodents, I opened up my business to family and friends with the contingency that they would continue to provide me updates and data about how their animal was doing. This allowed me to continue looking at other health conditions in relation to my diet. Over the past three years, I have had multiple clients test out the diet and see immediate improvements in their animal's health.
In 2016 I contacted the Pennsylvania Society of Biomedical Research to receive a grant for research specimens and materials for a seven-month-long research trial. I received sixteen female albino rats at 23 days old. The study's goal was to determine the effects of the core diet compared to a store-bought diet on the weight gain of rodentia. The rats were separated into four groups, two control and two experimental. Control group one consumed only my formulated diet, control group two consumed just the store-bought diet, experimental group one consumed my diet initially before transitioning to the store-bought diet, and experimental group two consumed the store-bought diet before transitioning to my diet. I measured weight gain and tail length three times a week throughout the seven months. After the project, the rodents who had eaten my diet were much larger than the rodents who consumed the store-bought diet. In the experimental groups, the rodents who switched to the store-bought food began to lose weight, and the growth rate slowed, while the rodents who switched to my diet increased in weight and growth rate.
In 2018 I wanted to recreate the previous year's trials with more specimens and tests to confirm further that my diet was not causing underlying health conditions. This year, it became prevalent for people to feed their animals vegan/vegetarian diets, so I added a vegan diet to the trials to determine the effects of that kind of diet on a herbivore. The project lasted four months and included twelve specimens split into three groups. Group one consumed my diet, group two consumed the store-bought diet, and group three consumed the vegan diet. Weight gain was measured every five days, and blood glucose concentrations were measured seven times throughout the project. Blood glucose was measured on the first day, three times at the midpoint of the project (before eating, one hour postprandial, and 8 hours postprandial), and three times after the project (once again before eating, one hour postprandial, and 8 hours postprandial). After the project, it could be concluded that my diet on average did not cause hyper or hypoglycemia, the vegan diet on average caused hypoglycemia, and the store-bought diet on average caused hyperglycemia.
If you would like more detailed information on the research conducted or to read the official research papers correlated with the Rodentia research please contact me.